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ATCC? Number:50502?
Organism: Encephalitozoon cuniculi Levaditi et al.
Designations: canine subtype species
Isolation: beagle puppy, Richmond, TX, 1978
Depositors: ES Didier
History: ATCC<<--ES Didier<<--J.A. Shadduck
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2313: RPMI 1640 medium ATCCmedium 722: Minimum essential medium (MEM)Temperature: 37.0°C Duration: 5% CO2 Protocol: ATCCNO: 50502 SPEC: Thaw ampule in a 37C water bath and aseptically transfer the contents to a T-25 tissue culture flask containing a monolayer of MDCK cells (ATCCCCL-34). Twice a week, remove the culture fluid and feed the infected cells with fresh medium. Centrifuge the culture fluid at 400-500 x g for 10 minutes, discard the supernatant, resuspend the cell pellet in fresh medium and transfer it to a fresh monolayer of host cells. Alternatively, transfer the culture fluid to a 15-ml plastic centrifuge tube and store at 4C in an upright position. The spores can be concentrated simply by allowing them to settle to the bottom of the tube. Remove the supernatant from the tube when the spores have settled. Using this technique the spores can be concentrated to high densities over a period of several months.
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Comments: Mycoplasma free. Inhibition of replication  [18380] Characterization of three strains  [18381] Dog isolate infecting AIDS patient  [18383] Treatment with Albendazole & Fumagillin  [18384] Molecular comparison with other microsporidian species  [18385]
References:  4930: Shadduck JA, et al. Isolation of the causative organism of canine encephalitozoonosis.  Vet. Pathol. 15: 449-460, 1978. PubMed: 695219 18380: Didier ES. Reactive nitrogen intermediates implicated in the inhibition of Encephalitozoon cunculi (phylum microspora) replication in murine peritoneal macrophages.  Parasite Immunol. 17: 405-412, 1995. PubMed: 7501421 18381: Didier ES, et al. Identification and characterization of three Encephalitozoon cuniculi strains.  Parasitology 111: 411-421, 1995. PubMed: 11023405 18383: Didier ES, et al. A microsporidian isolated from an AIDS patient corresponds to Encephalitozoon cuniculi III, originally isolated from domestic dogs.  J. Clin. Microbiol. 34: 2835-2837, 1996. PubMed: 8897194 18384: Didier ES, et al. Diagnosis of disseminated microsporidian Encephalitozoon hellum infection by PCR - southern analysis and successful treatment with albendazole and fumagillin.  J. Clin. Microbiol. 34: 947-952, 1996. PubMed: 8815114 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients.  J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 70345: Marshall MM, et al. Comparison of UV inactivation of spores of three encephalitozoon species with that of spores of two DNA repair-deficient Bacillus subtilis biodosimetry strains.  Appl. Environ. Microbiol. 69: 683-685, 2003. PubMed: 12514061 70348: Johnson CH, et al. Chlorine inactivation of spores of Encephalitozoon spp..  Appl. Environ. Microbiol. 69: 1325-1326, 2003. PubMed: 12571067 70447: Hester JD, et al. Species-specific detection of three human-pathogenic microsporidial species from the genus Encephalitozoon via fluorogenic 5' nuclease PCR assays.  Mol. Cell Probes 16: 435-444, 2002. PubMed: 12490145