ATCC? Number:50519?
Organism: Ministeria vibrans Tong
Isolation: seawater, Atlantic Ocean, Southampton, England, 1992 [31832]
Depositors: S Tong
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1525: Seawater 802 mediumTemperature: 25.0°C Protocol: ATCCNO: 50519 SPEC: The strain is routinely distributed as a frozen stabilate. See the general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing 10 ml of ATCCmedium 1525. The baterial flora that is present in the shipped culture will support growth. Growth may be improved by using bacterized ATCCmedium 1525 with a single species of bacteria. Bacterization is accomplished by inoculating the medium with Enterobacter aerogenes ATCC13048 approximately 24 hours before use. Other species of bacteria may work equally well but this must be empirically assessed. The culture is routinely passaged every 14 days. This strain does not form a cyst stage and can be lost if it is not transferred regularly. To subculture, agitate and aseptically transfer 0.25 ml to 10 ml of freshly bacterized medium in a T-25 flask. Screw the cap on tightly and incubate at 25C.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Type Strain: yes(type strain)
Classification: KINGDOM: Protozoa
References: 31832: Tong S. Heterotrophic flagellates and other protists from Southampton water, U.K.. Ophelia 47: 71-131, 1997. 71066: Cavalier-Smith T, Chao EE-Y. Phylogeny of choanozoa, apusozoa, and other protozoa and early eukaryote megaevolution. J. Mol. Evol. 56: 540-563, 2003. PubMed: 12698292