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平臺(tái)編號：bio-47487
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國際編號：ATCC 12716
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拉丁屬名： Euglena gracilis Klebs
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規(guī)格：Frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Euglena gracilis
Euglena gracilis Klebs
12716 ™
Product categoryProtists
Product typeAlgae
ClassificationExcavata, Euglenozoa, Euglenida, EugleneaStrain designationZType strainNoIsolation sourceFreshwater material from SaedeleerProduct formatFrozen
General
Specific applicationsassay of vitamin B12 cobalamin, cobalamins
Characteristics
CommentsMedium for Vitamin B12 assay
Regulation of cell shape
Growth and cell volume
Aromatic amino acid pathway
water-soluble vitamins in cells and spent culture supernatants
Molecular biology
Synchronization of division
cryopreservation
photosynthetic
Handling information
MediumATCC Medium 351: Hutner's medium for Euglena
Instruction for complete mediumATCC Medium 351Temperature25°C
Culture systemAxenicHandling procedureFrozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
1. To thaw a frozen ampule, aseptically add 0.5 ml fresh ATCC medium 351 broth to the frozen pellet, then place the ampule in a 35°C water bath until thawed (2-3 min). Immerse the ampule just sufficiently to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the entire contents to a single 16 x 125 mm screw-capped test tube containing 5 ml of ATCC medium 351 broth. Incubate the tube upright for one hour at 25°C.
3. Gently remove as much supernatant as possible (the methanol cryoprotectant can inhibit growth) and refill with an equal volume of fresh broth medium.
4. Incubate on a horizontal slant at 50-100 µEinsteins/m2/s irradiance at 25°C with the cap loosened one half turn. Maintain under a 14/10 h light-dark photoperiod.
Culture maintenance1. Inoculate a tube of fresh broth medium with 0.2 ml from a growing culture at or near peak density.
2. Incubate on a horizontal slant at 50-100 µEinsteins/m2/s irradiance at 25°C with the cap loosened one half turn. Maintain under a 14/10 h light-dark photoperiod.
