Leptospira interrogans (Stimson) Wenyon
43642 ™
Leptospira interrogans strain RGA was isolated in 1915 from human blood via guinea pig.
Product categoryBacteria
Strain designationRGAType strainYesIsolation sourceHuman blood via guinea pig; isolated by Uhlenhuth and FrommeApplicationsAgricultural research
Product formatFrozenStorage conditions-80°C or colder
General
PreceptrolNo
Characteristics
Serotypeicterohaemorrhagiae
Handling information
MediumATCC Medium 2653: Leptospira Medium
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature30°C
AtmosphereAerobic
Handling procedureTRANSFER IMMEDIATELY upon thawing by aseptically withdrawing cell suspension from vial and inoculating a 10-12 mL tube of Medium #2653, inserting the pipette just below the surface of the semi-solid medium. Aliquots of 0.5 mL from this tube may be used to inoculate additional tubes if needed. An aerobic blood plate may also be streaked to test for purity.
Incubate test tubes with screw caps lightly tightened along with the blood plate at 30°C under aerobic conditions.
Initial growth may take up to 7 days. Observe growth as a thin, tight band forming just below the surface of the medium. The band thickens as incubation continues.
The aerobic blood plate should show no signs of growth.
Handling notesAdditional information on this culture is available on the ATCC® web site at www.atcc.org.
