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ATCC? Number:50505?
Organism: Vittaforma corneae (Shadduck et al.) Silveira and Canning
Isolation: corneal biopsy material from HIV-seronegative adult human male with keratitis, 1989
Depositors: ES Didier
History: ATCC<<--ES Didier<<--J.A. Shadduck
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2313: RPMI 1640 medium ATCCmedium 722: Minimum essential medium (MEM)Temperature: 37.0°C Duration: 5% CO2 Protocol: ATCCNO: 50502 SPEC: Thaw ampule in a 37C water bath and aseptically transfer the contents to a T-25 tissue culture flask containing a monolayer of MDCK cells (ATCCCCL-34). Twice a week, remove the culture fluid and feed the infected cells with fresh medium. Centrifuge the culture fluid at 400-500 x g for 10 minutes, discard the supernatant, resuspend the cell pellet in fresh medium and transfer it to a fresh monolayer of host cells. Alternatively, transfer the culture fluid to a 15-ml plastic centrifuge tube and store at 4C in an upright position. The spores can be concentrated simply by allowing them to settle to the bottom of the tube. Remove the supernatant from the tube when the spores have settled. Using this technique the spores can be concentrated to high densities over a period of several months.
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Comments: Contaminated with mycoplasma. Molecular comparison with other microspordian species  [18381]  [18385] Molecular comparison of isolates from different animals  [18383] Discrimination between different species  [18384]
References:  18381: Didier ES, et al. Identification and characterization of three Encephalitozoon cuniculi strains.  Parasitology 111: 411-421, 1995. PubMed: 11023405 18383: Didier ES, et al. A microsporidian isolated from an AIDS patient corresponds to Encephalitozoon cuniculi III, originally isolated from domestic dogs.  J. Clin. Microbiol. 34: 2835-2837, 1996. PubMed: 8897194 18384: Didier ES, et al. Diagnosis of disseminated microsporidian Encephalitozoon hellum infection by PCR - southern analysis and successful treatment with albendazole and fumagillin.  J. Clin. Microbiol. 34: 947-952, 1996. PubMed: 8815114 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients.  J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 23787: Shadduck JA, et al. Isolation of a microsporidian from a human patient.  J. Infect. Dis. 162: 773-776, 1990. PubMed: 2117629 70540: Mittleider D, et al. Sequence survey of the genome of the opportunistic microsporidian pathogen, Vittaforma corneae.  J. Eukaryot. Microbiol. 49: 393-401, 2002. PubMed: 12425527