-
平臺編號：bio-47501
-
國際編號：ATCC 50493
-
拉丁屬名： Acanthamoeba castellanii
-
規(guī)格：frozen
-
用途：ATCC原裝進(jìn)口
-
服務(wù)費(fèi)用：
加載中…… - 訂購
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Acanthamoeba castellanii
ATCC? Number:50493?
Organism: Acanthamoeba castellanii (Douglas) Page
Designations: CDC:0786:V042
Isolation: human cornea, Illinois, 1986
Depositors: RJ Gast, TJ Byers
History: ATCC<<--RJ Gast, TJ Byers<<--G.S. Visvesvara
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30135 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Subgenus systematics based upon SSU rDNA sequence data [21640] Axenic
Cross References: Nucleotide (GenBank) : U07403 nuclear SSU rRNA gene
Classification: KINGDOM: Protozoa
References: 21640: Gast RJ, et al. Subgenus systematics of Acanthamoeba: Four nuclear 18S rDNA sequence types. J. Eukaryot. Microbiol. 43: 498-504, 1996. PubMed: 8976608 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 43732: Stothard DR, et al. Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type. J. Clin. Microbiol. 37: 2687-2693, 1999. PubMed: 10405422 70805: Ledee DR, et al. Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 44: 1142-1149, 2003. PubMed: 12601042
ATCC50493
Organism: Acanthamoeba castellanii (Douglas) Page
Designations: CDC:0786:V042
Isolation: human cornea, Illinois, 1986
Depositors: RJ Gast, TJ Byers
History: ATCC<<--RJ Gast, TJ Byers<<--G.S. Visvesvara
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30135 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Subgenus systematics based upon SSU rDNA sequence data [21640] Axenic
Cross References: Nucleotide (GenBank) : U07403 nuclear SSU rRNA gene
Classification: KINGDOM: Protozoa
References: 21640: Gast RJ, et al. Subgenus systematics of Acanthamoeba: Four nuclear 18S rDNA sequence types. J. Eukaryot. Microbiol. 43: 498-504, 1996. PubMed: 8976608 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 43732: Stothard DR, et al. Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type. J. Clin. Microbiol. 37: 2687-2693, 1999. PubMed: 10405422 70805: Ledee DR, et al. Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 44: 1142-1149, 2003. PubMed: 12601042
ATCC50493
