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平臺編號：bio-47502
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國際編號：ATCC 50507
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拉丁屬名： Encephalitozoon intestinalis
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Encephalitozoon intestinalis
ATCC? Number:50507?
Organism: Encephalitozoon intestinalis (Cali et al.) Hartskeerl et al.
Designations: nasal isolate
Isolation: nasal material from HIV-seropositive human, New York City, NY, 1994
Depositors: ES Didier
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2313: RPMI 1640 medium ATCCmedium 722: Minimum essential medium (MEM)Temperature: 37.0°C Duration: 5% CO2 Protocol: ATCCNO: 50502 SPEC: Thaw ampule in a 37C water bath and aseptically transfer the contents to a T-25 tissue culture flask containing a monolayer of MDCK cells (ATCCCCL-34). Twice a week, remove the culture fluid and feed the infected cells with fresh medium. Centrifuge the culture fluid at 400-500 x g for 10 minutes, discard the supernatant, resuspend the cell pellet in fresh medium and transfer it to a fresh monolayer of host cells. Alternatively, transfer the culture fluid to a 15-ml plastic centrifuge tube and store at 4C in an upright position. The spores can be concentrated simply by allowing them to settle to the bottom of the tube. Remove the supernatant from the tube when the spores have settled. Using this technique the spores can be concentrated to high densities over a period of several months.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Mycoplasma free.
References: 24196: Doultree JC, et al. In vitro growth of the microsporidian Septata intestinalis from an AIDS patient with disseminated illness. J. Clin. Microbiol. 33: 463-470, 1995. PubMed: 7714208
Organism: Encephalitozoon intestinalis (Cali et al.) Hartskeerl et al.
Designations: nasal isolate
Isolation: nasal material from HIV-seropositive human, New York City, NY, 1994
Depositors: ES Didier
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2313: RPMI 1640 medium ATCCmedium 722: Minimum essential medium (MEM)Temperature: 37.0°C Duration: 5% CO2 Protocol: ATCCNO: 50502 SPEC: Thaw ampule in a 37C water bath and aseptically transfer the contents to a T-25 tissue culture flask containing a monolayer of MDCK cells (ATCCCCL-34). Twice a week, remove the culture fluid and feed the infected cells with fresh medium. Centrifuge the culture fluid at 400-500 x g for 10 minutes, discard the supernatant, resuspend the cell pellet in fresh medium and transfer it to a fresh monolayer of host cells. Alternatively, transfer the culture fluid to a 15-ml plastic centrifuge tube and store at 4C in an upright position. The spores can be concentrated simply by allowing them to settle to the bottom of the tube. Remove the supernatant from the tube when the spores have settled. Using this technique the spores can be concentrated to high densities over a period of several months.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Mycoplasma free.
References: 24196: Doultree JC, et al. In vitro growth of the microsporidian Septata intestinalis from an AIDS patient with disseminated illness. J. Clin. Microbiol. 33: 463-470, 1995. PubMed: 7714208
