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平臺編號：bio-47522
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國際編號：ATCC 50456
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拉丁屬名： Stephanoeca diplocostata
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Stephanoeca diplocostata
ATCC? Number:50456?
Organism: Stephanoeca diplocostata Ellis
Isolation: rockpool at Le Cabellou near Concarneau, South Brittany, France, 1976
Depositors: BS Leadbeater
Biosafety Level:1
Shipped: frozen
Growth Conditions: Temperature: 15.0°C Duration: grown with mixed bacteria Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCCmedium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCCmedium 1361 in a T-25 flask.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Developmental studies [23919] Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 [16172870]
Classification: KINGDOM: Protozoa
References: 4667: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis. V. The cytoskeleton and the effects of microtubule poisons. Protoplasma 136: 1-15, 1987. 4729: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis VI. Effects of silica replenishment on silica impovershed cells. Protoplasma 153: 71-84, 1989. 4944: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis I. Ultrastructure of the non-dividing cell and costal strip production. Protoplasma 98: 241-262, 1979. 5031: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostat Ellis IV. Effects of silica deprivation on growth and lorica production. Protoplasma 127: 171-179, 1985. 23919: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis. VII. Dynamics of costal strip accumulation and lorica assembly. Eur. J. Protistol. 30: 111-124, 1994. 16172870: Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774
Organism: Stephanoeca diplocostata Ellis
Isolation: rockpool at Le Cabellou near Concarneau, South Brittany, France, 1976
Depositors: BS Leadbeater
Biosafety Level:1
Shipped: frozen
Growth Conditions: Temperature: 15.0°C Duration: grown with mixed bacteria Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCCmedium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCCmedium 1361 in a T-25 flask.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Developmental studies [23919] Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 [16172870]
Classification: KINGDOM: Protozoa
References: 4667: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis. V. The cytoskeleton and the effects of microtubule poisons. Protoplasma 136: 1-15, 1987. 4729: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis VI. Effects of silica replenishment on silica impovershed cells. Protoplasma 153: 71-84, 1989. 4944: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis I. Ultrastructure of the non-dividing cell and costal strip production. Protoplasma 98: 241-262, 1979. 5031: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostat Ellis IV. Effects of silica deprivation on growth and lorica production. Protoplasma 127: 171-179, 1985. 23919: Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis. VII. Dynamics of costal strip accumulation and lorica assembly. Eur. J. Protistol. 30: 111-124, 1994. 16172870: Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774
