ATCC? Number:50453?
Organism: Choanoeca perplexa Ellis
Isolation: Atlantic Ocean off Plymouth, England, 1977
Depositors: BS Leadbeater
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1361: Marine flagellate mediumTemperature: 15.0°C Duration: grown with mixed bacteria Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCCmedium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCCmedium 1361 in a T-25 flask.
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Comments: Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 [16172870]
Classification: KINGDOM: Protozoa
References: 4998: Leadbeater BS. . J. Mar. Biol. Assoc. U.K. 57: 285-301, 1977. 16172870: Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774