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ATCC? Number:50451?
Organism: Encephalitozoon hellem Didier et al.
Designations: CDC:0291:V213
Isolation: urine from adult human male AIDS patient, Georgia, 1991
Depositors: GS Visvesvara
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Duration: 5% CO2 Protocol: ATCCNO: 50451 SPEC: Thaw ampule in a 35C water bath and aseptically transfer contents to a T-25 tissue culture flask containing a monolayer of lung fibroblasts (ATCCCCL-75). Twice per week, remove the culture fluid and refeed the culture with fresh medium. Centrifuge the culture fluid at 1300 x g for 10 minutes, discard the supernatant, and transfer the resuspended cell pellet to a fresh monolayer of lung fibroblasts.
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Comments: Control strain for enterocytozoan identification  [43276]  [43277]  [43280]  [43278] detection of drug-induced effects in infected green monkey kidney cells  [48251]  [47325] Western blot and immunofluorescence analysis  [38797] Culture, electron microscopy, and immunobolt studies  [5042] Inhibition of spore polar filament extrusion  [4993] Use of rabbit antiserum conspecific for Encephalitozoon hellum for identification  [21634] Polyclonal and monoclonal antibody and PCR based identification  [21635] Serologic and molecular identification  [21636] Molecular PCR based identification  [21637]
References:  4993: Leitch GJ, et al. Inhibition of the spore polar filament extrusion of the microsporidium, Encephalitozoon hellem, isolated from an AIDS patient.  J. Eukaryot. Microbiol. 40: 711-717, 1993. PubMed: 8292991 5042: Visvesvara GS, et al. Culture, electron microscopy, and immunoblot studies on a microsporidian parasite isolated from the urine of a patient with AIDS.  J. Protozool. 38: 105S-111S, 1991. PubMed: 1818126 21634: Diesenhouse MC, et al. Treatment of microsporidial keratoconjunctivitis with topical fumagillin.  Am. J. Opthalmol. 115: 293-298, 1993. PubMed: 8117342 21635: Visvesvara GS, et al. Polyclonal and monoclonal antibody and PCR-amplified small-subunit rRNA identification of a microsporidian, Encephalitozoon hellem, isolated from an AIDS patient with disseminated infection.  J. Clin. Microbiol. 32: 2760-2768, 1994. PubMed: 7852569 21636: Visvesvara GS, et al. In vitro culture and serologic and molecular identification of Septata intestinalis isolated from urine of a patient with AIDS.  J. Clin. Microbiol. 33: 930-936, 1995. PubMed: 7790463 21637: De Groote MA, et al. Polymerase chain reaction and culture confirmation of disseminated Encephalitozoon cuniculi in a patient with AIDS: Successful therapy with albendazole.  J. Infect. Dis. 171: 1375-1378, 1995. PubMed: 7751721 32928: Gatti S, et al. Extraintestinal microsporidiosis in AIDS patients: clinical features and advanced protocols for diagnosis and characterization of the isolates.  J. Eukaryot. Microbiol. 44: 79S, 1997. PubMed: 9508459 38797: Croppo GP, et al. Western blot and immunofluorescence analysis of a human isolate of Encephalitozoon cuniculi established in culture from the urine of a patient with AIDS.  J. Parasitol. 83: 66-69, 1997. PubMed: 9057698 39769: Moura H, et al. Detection by an immunofluorescence test of Encephalitozoon intestinalis spores in routinely formalin-fixed stool samples stored at room temperature.  J. Clin. Microbiol. 37: 2317-2322, 1999. PubMed: 10364604 43276: Croppo GP, et al. Identification of the microsporidian Encephalitozoon hellem using immunoglobulin G monoclonal antibodies.  Arch. Pathol. Lab. Med. 122: 182-186, 1998. PubMed: 9499364 43277: del Aguila C, et al. Ultrastructure, immunofluorescence, western blot, and PCR analysis of eight isolates of Encephalitozoon (Septata) intestinalis established in culture from sputum and urine samples and duodenal aspirates of five patients with AIDS.  J. Clin. Microbiol. 36: 1201-1208, 1998. PubMed: 9574677 43278: Bornay-Llinares FJ, et al. Immunologic, microscopic, and molecular evidence of Encephalitozoon intestinalis (Septata intestinalis) infection in mammals other than humans.  J. Infect. Dis. 178: 820-826, 1998. PubMed: 9728552 43280: Scaglia M, et al. Asymptomatic respiratory tract microsporidiosis due to Encephalitozoon hellem in three patients with AIDS.  Clin. Infect. Dis. 26: 174-176, 1998. PubMed: 9455527 47325: Leitch GJ, et al. Use of fluorescent probe to assess the activities of candidate agents against intracellular forms of Encephalitozoon microsporidia.  Antimicrob. Agents Chemother. 41: 337-344, 1997. PubMed: 9021189 48251: He Q, et al. Effects of nifedipine, metronidazole, and nitric oxide donors on spore germination and cell culture infection of the microsporidia Encephalitozoon hellem and Encephalitozoon intestinalis.  Antimicrob. Agents Chemother. 40: 179-185, 1996. PubMed: 8787902 70345: Marshall MM, et al. Comparison of UV inactivation of spores of three encephalitozoon species with that of spores of two DNA repair-deficient Bacillus subtilis biodosimetry strains.  Appl. Environ. Microbiol. 69: 683-685, 2003. PubMed: 12514061 70346: Xiao L, et al. Genotyping Encephalitozoon hellem isolates by analysis of the polar tube protein gene.  J. Clin. Microbiol. 39: 2191-2196, 2001. PubMed: 11376056 70347: del Aguila C, et al. In vitro culture, ultrastructure, antigenic, and molecular characterization of Encephalitozoon cuniculi isolated from urine and sputum samples from a Spanish patient with AIDS.  J. Clin. Microbiol. 39: 1105-1108, 2001. PubMed: 11230434 70348: Johnson CH, et al. Chlorine inactivation of spores of Encephalitozoon spp..  Appl. Environ. Microbiol. 69: 1325-1326, 2003. PubMed: 12571067 70447: Hester JD, et al. Species-specific detection of three human-pathogenic microsporidial species from the genus Encephalitozoon via fluorogenic 5' nuclease PCR assays.  Mol. Cell Probes 16: 435-444, 2002. PubMed: 12490145