ATCC? Number:50428?
Organism: Acanthamoeba lenticulata Molet and Ermolieff-Braun
Designations: JC-1
Isolation: sewage-contaminated sediment, Jones Creek, Long Island, NY, 1984
Depositors: TK Sawyer
Biosafety Level:1
Shipped: freeze-dried
Growth Conditions: ATCCmedium 712: PYGMax Temperature: 35.0°C Min Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30010 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
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Comments: Intron in SSU rRNA gene has been sequenced in the laboratory of T.J. Byers, Ohio State University
Classification: KINGDOM: Protozoa
References: 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 70302: Schroeder-Diedrich JM, et al. Group-I introns with unusual sequences occur at three sites in nuclear 18S rRNA genes of Acanthamoeba lenticulata. Curr. Genet. 34: 71-78, 1998. PubMed: 9683678