ATCC? Number:50433?
Organism: Trypanoplasma borreli Laveran and Mesnil
Designations: Pg-JH
Isolation: axenic clone derived from gut of leech, Piscicola geometra, Southern Bohemia, Czech Republic, 1990
Depositors: D Maslov
History: ATCC<<--D Maslov<<--J. Lom
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1029: LIT mediumTemperature: 15.0°C Duration: axenic Protocol: ATCCNO: 50433 SPEC: Upon arrival place frozen ampule directly into a 35C water bath, transfer thawed contents to 10 ml of fresh medium in a T-25 tissue culture flask. Maintain by transferring an inoculum of 0.5-1.0 ml to a fresh flask of medium every 4-5 days. Slow agitation of the culture will improve growth.
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Comments: RNA editing and mitochondrial genomic organization [24082]
Cross References: Nucleotide (GenBank) : U39581 Trypanoplasma borreli large subunit ribosomal RNA.
References: 4869: Peckova H, Lom J. Growth, morphology and division of flagellates of the genus Trypanoplasma (Protozoa, Kinetoplastida) in vitro. Parasitol. Res. 76: 553-558, 1990. PubMed: 2217113 24082: Maslov DA, Simpson L. RNA editing and mitochondrial genomic organization in the cryptobiid kinetoplastid protozoan Trypanoplasma borreli. Mol. Cell. Biol. 14: 8174-8182, 1994. PubMed: 7969154 38759: Maslov DA, et al. Phylogeny of trypanosomes as inferred from the small and large subunit rRNAs: implications for the evolution of parasitism in the trypanosomatid protozoa. Mol. Biochem. Parasitol. 75: 197-205, 1996. PubMed: 8992318