>美國(guó)ATCC>ATCC菌種類(lèi)>Acanthamoeba pearcei
ATCC® Number:50436™
Organism: Acanthamoeba pearcei Nerad et al.
Designations: 205-1-AX
Isolation: axenic clone derived from the type strain, ATCC50435, 1994
Depositors: TK Sawyer
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0℃ Duration: axenic Protocol: ATCCNO: 50436 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to 5.0 ml of ATCCmedium 712 in a 16 x 125 mm screw-capped plastic test tube. Screw the cap on tightly and incubate on a 15 degree horizontal slant at 25C. Trophozoites should attach to the bottom surface of the test tube. Within 7-10 days the trophozoites will form the equivalent of a monolayer on the bottom of the tube. To subculture, vigorously agitate the tube and aseptically transfer a 0.1-ml aliquot to a fresh tube of medium. Incubate as above. Note: The transfer interval will depend on the quality of the medium, the size of the vessel, the volume of the medium, and the inoculum size. When cultivated at ATCCat 25C in plastic 16 x 125 mm screw-capped test tubes (with the caps screwed on tightly) containing 5 ml of medium 712, the transfer interval is every 2 weeks when the inoculum is 0.1 ml. Cells remain viable for longer periods in plastic than in glass test tubes.
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Classification: KINGDOM: Protozoa