ATCC? Number:50418?
Organism: Cercomonas plasmodialis Mylnikov
Designations: AZ-6
Isolation: plant (dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992)
Depositors: TK Sawyer
History: ATCC<<--TK Sawyer<<--T.A. Nerad
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Duration: grown with Enterobacter aerogenes ATCC13048 and mixed bacteria Protocol: Once the culture is established, the cysts will excyst and the trophozoites will comsume the bacteria in the medium. When the bacterial numbers have been reduced below a certain level, the Cercomonas will once again encyst. The cysts are stabile for at least 1-2 months. Subculture by detaching the cysts from the surface of the flask with a sterile cell scrapper, agitate, and aseptically transfer a 0.1-ml aliquot to a fresh flask containing 10 ml of bacterized medium 802.
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Comments: This is the only species of this genus that produces a microplasmodium. The microplasmodium gives rise to large multinucleate cysts. dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992.
Classification: KINGDOM: Protozoa