ATCC? Number:50388?
Organism: Acanthamoeba stevensoni Sawyer et al.
Designations: RB-F-1
Isolation: sewage-contaminated hard clam, Mercenaria mercenaria, beds in Raritan Bay, NY, near the Staten Island shoreline
Depositors: TK Sawyer
Biosafety Level:2
Shipped: freeze-dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Max Temperature: 35.0°C Min Temperature: 25.0°C Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Type Strain: yes(type strain)
Comments: species description [20724]
Cross References: Nucleotide (GenBank) : AF019069 Acanthamoeba stevensoni RB:F:1 18S ribosomal RNA gene, partial sequence.
Classification: KINGDOM: Protozoa
References: 20724: Sawyer TK, et al. Acanthamoeba stevensoni n. sp. (protozoa: amoebida) from sewage-contaminated shellfish beds in Raritan Bay, New York. J. Eukaryot. Microbiol. 40: 742-746, 1993. 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 57068: Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331