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平臺編號：bio-47595
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國際編號：ATCC 50374
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拉丁屬名： Acanthamoeba castellanii
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規(guī)格：freeze-dried
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Acanthamoeba castellanii*
ATCC® Number:50374™
Organism: Acanthamoeba castellanii (Douglas) Page
Designations: AC30/Castellanii
Isolation: Cryptococcus sp. culture, London, 1930
Depositors: TJ Byers, RJ Gast
History: ATCC<<--TJ Byers, RJ Gast<<--E. Willaert
Biosafety Level:1
Shipped: freeze-dried
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30010 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
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Comments: May be equivalent to ATCC30234. Subgenus systematics based upon SSU rDNA sequence data [21640]
Cross References: Nucleotide (GenBank) : U07413 nuclear SSU rRNA gene
Classification: KINGDOM: Protozoa
References: 21640: Gast RJ, et al. Subgenus systematics of Acanthamoeba: Four nuclear 18S rDNA sequence types. J. Eukaryot. Microbiol. 43: 498-504, 1996. PubMed: 8976608 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 70805: Ledee DR, et al. Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 44: 1142-1149, 2003. PubMed: 12601042
