>美國(guó)ATCC>ATCC菌種類>RS/18A
ATCC? Number:50316?
Organism: Cercomonas sp. 2
Designations: RS/18A
Isolation: soil/litter from polar bear enclosure, Chester Zoo, Chester, England,?
Depositors: T Cavalier-Smith
History: ATCC<<--T Cavalier-Smith<<--A.J. Cowling
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 1967: Soil freshwater cereal grass mediumTemperature: 25.0°C Protocol: ATCCNO: 50315 SPEC: This strain is shipped as a growing culture. Agitate the test tube and distribute the entire contents to 10 ml of fresh ATCCmedium 1967 bacterized with Enterobacter aerogenes ATCC13048. Screw the cap on tightly and incubate at 25C. This strain does not form a cyst stage and must be passaged at regular intervals of approximately 14 days. To subculture, agitate the flask vigorously and aseptically transfer a 0.25-ml aliquot to 10 ml of freshly bacterized ATCCmedium 1967. Incubate as above.
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Comments: The SSU rRNA gene sequence of ATCC50316 is quite distinct from that of ATCC50317, ATCC50318, or ATCC50319. These are probably different species and are designated as such by the different suffixes. phylogeny based on SSU rDNA sequence [21778]
Cross References: Nucleotide (GenBank) : AF119173 Cercomonas ATCC50316 beta-tubulin (btub) gene, partial cds.Nucleotide (GenBank) : U42448 nuclear SSU rRNA gene
Classification: KINGDOM: Protozoa
References: 21778: Cavalier-Smith T, Chao EE. Sarcomonad ribosomal RNA sequences, rhizopod phylogeny, and the origin of euglyphid amoebae. Arch. Protistenkd. 147: 227-236, 1997. 70182: Archibald JM, et al. A novel polyubiquitin structure in cercozoa and foraminifera: evidence for a new eukaryotic supergroup. Mol. Biol. Evol. 20: 62-66, 2003. PubMed: 12519907