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平臺編號：bio-47684
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國際編號：ATCC 50268
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拉丁屬名： Hexamita inflata
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Hexamita inflata
ATCC? Number:50268?
Organism: Hexamita inflata Dujardin
Designations: AZ-4
Isolation: fibrous plant roots and soil from small pond, Desert Museum, Tucson, AZ, 1991
Depositors: TK Sawyer
History: ATCC<<--TK Sawyer<<--T.A. Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1773: Hexamita mediumTemperature: 25.0°C Duration: anaerobic Protocol: ATCCNO: 50268 SPEC:This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to a 16 x 125 mm screw-capped test tube containing 12 ml of bacterized ATCCmedium 1773, screw the cap on tightly, and incubate on a 15 degree slant at 25C. Trophozoites should be active on the following day. Within 7-10 days most of the cells will encyst. The cysts remain viable for at least one month. However, to ensure viability, subculture every 14-21 days. To subculture, rub the surface of the tube with a sterile cotton swab, gently invert 10 times, aseptically transfer a 0.25-ml aliquot to a fresh tube of bacterized ATCCmedium 1773, and incubate as above. This strain has been cultivated with a non-pathogenic strain of Enterobacter aerogenes ATCC-13048 as a food source. Other unidentified bacteria are also present. Note: ATCCmedium 1773 is bacterized by inoculating with a partial bacteriological loopful of a non-pathogenic strain of K. pneumoniae subsp. pneumoniae ATCC-700831 (other strains of Klebsiella will probably work equally well) from an ATCCmedium 3 slant approximately 24 hours before using. There is currently no known commercial source of rice starch that is suitable for inclusion in ATCCmedium 1773. However, purified rice starch of a suitable nature can be prepared (Diamond, L.S. Lumen dwelling protozoa: Entamoeba, trichomonads, and Giardia. In: Jensen, J.B. ed., In vitro cultivation of protozoan parasites. Boca Raton, FL: CRC Press; 1983:p. 75).
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Structure/phylogeny of glyceraldehyde-3-phosphate dehydrogenase genes [21695] Small subunit ribosomal RNA [24150]
Cross References: Nucleotide (GenBank) : AF022888 Hexamita inflata dynein heavy chain homolog (hdyhh) gene, complete cds.Nucleotide (GenBank) : U40817 Hexamita inflata glyceraldehyde-3-phosphate dehydrogenase gene, partial cds.Nucleotide (GenBank) : L07836 Hexamita inflata small subunit (16S-like) ribosomal RNA sequence.
References: 21695: Rozario C, et al. Primary structure and phylogenetic relationships of glyceraldehyde-3-phosphate dehydrogenase genes of free-living and parasitic diplomonad flagellates. J. Eukaryot. Microbiol. 43: 330-340, 1996. PubMed: 8768438 24150: Leipe DD, et al. Small subunit ribosomal RNA+ of Hexamita inflata and the quest for the first branch in the eukaryotic tree. Mol. Biochem. Parasitol. 59: 41-48, 1993. PubMed: 8515782 33564: Chen N, et al. A dynein heavy chain homologue gene in Hexamita inflata. Gene 208: 83-87, 1998. PubMed: 9479053
ATCC50268
Organism: Hexamita inflata Dujardin
Designations: AZ-4
Isolation: fibrous plant roots and soil from small pond, Desert Museum, Tucson, AZ, 1991
Depositors: TK Sawyer
History: ATCC<<--TK Sawyer<<--T.A. Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1773: Hexamita mediumTemperature: 25.0°C Duration: anaerobic Protocol: ATCCNO: 50268 SPEC:This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to a 16 x 125 mm screw-capped test tube containing 12 ml of bacterized ATCCmedium 1773, screw the cap on tightly, and incubate on a 15 degree slant at 25C. Trophozoites should be active on the following day. Within 7-10 days most of the cells will encyst. The cysts remain viable for at least one month. However, to ensure viability, subculture every 14-21 days. To subculture, rub the surface of the tube with a sterile cotton swab, gently invert 10 times, aseptically transfer a 0.25-ml aliquot to a fresh tube of bacterized ATCCmedium 1773, and incubate as above. This strain has been cultivated with a non-pathogenic strain of Enterobacter aerogenes ATCC-13048 as a food source. Other unidentified bacteria are also present. Note: ATCCmedium 1773 is bacterized by inoculating with a partial bacteriological loopful of a non-pathogenic strain of K. pneumoniae subsp. pneumoniae ATCC-700831 (other strains of Klebsiella will probably work equally well) from an ATCCmedium 3 slant approximately 24 hours before using. There is currently no known commercial source of rice starch that is suitable for inclusion in ATCCmedium 1773. However, purified rice starch of a suitable nature can be prepared (Diamond, L.S. Lumen dwelling protozoa: Entamoeba, trichomonads, and Giardia. In: Jensen, J.B. ed., In vitro cultivation of protozoan parasites. Boca Raton, FL: CRC Press; 1983:p. 75).
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Structure/phylogeny of glyceraldehyde-3-phosphate dehydrogenase genes [21695] Small subunit ribosomal RNA [24150]
Cross References: Nucleotide (GenBank) : AF022888 Hexamita inflata dynein heavy chain homolog (hdyhh) gene, complete cds.Nucleotide (GenBank) : U40817 Hexamita inflata glyceraldehyde-3-phosphate dehydrogenase gene, partial cds.Nucleotide (GenBank) : L07836 Hexamita inflata small subunit (16S-like) ribosomal RNA sequence.
References: 21695: Rozario C, et al. Primary structure and phylogenetic relationships of glyceraldehyde-3-phosphate dehydrogenase genes of free-living and parasitic diplomonad flagellates. J. Eukaryot. Microbiol. 43: 330-340, 1996. PubMed: 8768438 24150: Leipe DD, et al. Small subunit ribosomal RNA+ of Hexamita inflata and the quest for the first branch in the eukaryotic tree. Mol. Biochem. Parasitol. 59: 41-48, 1993. PubMed: 8515782 33564: Chen N, et al. A dynein heavy chain homologue gene in Hexamita inflata. Gene 208: 83-87, 1998. PubMed: 9479053
ATCC50268
