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Chlorella sp. 拉丁名

(ATCC? 50258?) 統(tǒng)一編號

Strain Designations 菌種別名 NC64A 

Application 用途 Biofuel production 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Isolation 分離源 Not available 

Product Format 提供形式 frozen 

Storage Conditions 保存方法 

Frozen Cultures 冷凍物 : -70°C for 1 week; liquid N2 vapor for long term storage 

Freeze-dried Cultures 凍干物 : 2-8°C 

Live Cultures 活菌 : See Protocols section for handling information 

Type Strain 模式菌株 no 

Comments 注釋 Virus infection Growth cycle of a virus, PBCV-1 DNA synthesis following 

Infection with the Virus PBCV-1 Viruses of symbiotic 

Chlorella-like algae 

Lytic viruses 

Assembly site of the virus PBCV-1 

Ultrastructure studies of PBCV-1 virus 

Comparison of viruses Structural proteins and lipids of PBCV-1 virus 

Characterization of viruses 

Characterization of gene encoding the DNA methyltransferase from virus NC_1A

Medium 培養(yǎng)基 ATCC? Medium 5: Sporulation agar 

ATCC? Medium 847: Algal proteose agar 

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 20°C to 25°C 

Culture System: Axenic 

Cryopreservation Harvest and Preservation Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. 

Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium. 

While cells are centrifuging prepare a 10% (v/v) solution of sterile methanol in fresh medium. 

Mix the cell preparation and the 10% methanol in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 5% (v/v) Methanol. 

The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min. 

Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 

Place the vials in a controlled rate freezing unit. 

From room temperature cool at -1°C/min to -40°C. 

If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. 

At -40°C plunge into liquid nitrogen. 

Alternatively, place the vials in a Nalgene 1°C freezing apparatus. 

Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.) 

The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. 

Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C. 

To establish a culture from the frozen state place an ampule in a water bath set at 35°C. 

Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial. 

Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a centrifuge tube containing 5 mL of ATCC medium 5 (or ATCC medium 847) without agar. 

Centrifuge at 300 x g for 5 min. Remove most of the supernatant (=methanol, which can inhibit growth) and then resuspend the pellet. 

Transfer the culture to a 16 x 125 mm screw-capped test tube containing 5 mL of ATCC medium 5 broth (or ATCC medium 847 broth), or to the surface of an ATCC medium 5 agar plate (20 x 100 mm Petri plate containing 20 mL of ATCC medium 5 agar). (Alternately, an ATCC medium 847 agar plate can be used; this is a 20 x 100 mm Petri plate containing 20 mL of ATCC medium 847 agar.) 

Incubate the culture at 50-100 μEinsteins/m2/s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod.

Name of Depositor 寄存人 J Van Etten 

References 參考文獻(xiàn) Robock A. Virus infection of culturable Chlorella-like algae and development of a plaque assay. Science 219: 994-996, 1982. 

Van Etten JL, et al. Growth cycle of a virus, PBCV-1, tat infects Chlorella-like algae. Virology 126: 117-125, 1983. 

Van Etten JL, et al. DNA synthesis in a Chlorella-like alga following infection with the virus PBCV-1. Virology 134: 443-449, 1984. 

Van Etten JL, et al. Viruses of symbiotic Chlorella-like algae isolated from Paramecium bursaria and Hydra viridis. Proc. Natl. Acad. Sci. USA 79: 3867-3871, 1982. 

Van Etten JL, et al. Lytic viruses infecting a Chlorella-like alga. Virology 140: 135-143, 1985. PubMed: 2981448 

Meints RH, et al. Assembly site of the virus PBCV-1 in a Chlorella-like green alga: ultrastructural studies. Virology 154: 240-245, 1986. PubMed: 3750845 

Meints RH, et al. Infection of a Chlorella-like alga with the virus, PBCV-1: ultrastructural studies. Virology 138: 341-346, 1984. PubMed: 6495652 

Reisser W, et al. A comparison of viruses infecting two different Chlorella-like green algae. Virology 167: 143-149, 1988. PubMed: 2847410 

Skrdla MP, et al. Structural proteins and lipids in a virus, PBCV-1, which replicates in a Chlorella-like alga. Virology 135: 308-315, 1984. PubMed: 6740941 

Schuster AM, et al. Characterization of viruses infecting a eukaryotic Chlorella-like green alga. Virology 150: 170-177, 1986. PubMed: 3006334 

Narva KE, et al. Molecular cloning and characterization of the gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A. Nucleic Acids Res. 15: 9807-9823, 1987. PubMed: 3320956