ATCC? Number:50243?
Organism: Comandonia operculata Pernin and Pussard
Designations: CDC-149
Isolation: hospital cooling tower, Sioux Falls, SD, 1988
Depositors: BS Fields, TK Sawyer
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Classification: KINGDOM: Protozoa