ATCC? Number:50181?
Organism: Plasmodium berghei Vincke and Lips
Designations: NK65D
Isolation: derived from M. Yoeli strain NK65 by mosquito passage, Univ. Illinois, Urbana, pre-1969
Depositors: NE Alger
Biosafety Level:1
Shipped: frozen
Growth Conditions: Duration: in vivo, mouse Protocol: ATCCNO: 50173 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject the entire thawed contents of the ampule intraperitoneally into a 6- to 9-week-old mouse. The strain should be monitored, especially after infecting with thawed material. To infect additional mice, draw a drop of tail blood into a 1.0 ml syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat.
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Comments: Malaria vaccine antigen [4702] [4705] Isolation of a vaccine fraction [5060] Variation in virulence [4769] Malaria resistance [5051]
Classification: KINGDOM: Protozoa
References: 4702: Boyer JM, et al. Lipid composition and activity of a lytic factor isolated from Plasmodium berghei. Infect. Immun. 25: 805-809, 1979. PubMed: 387594 4705: D'Antonio LE, et al. Malaria vaccine antigen(s): detergent solubilization, partial isolation, and recovery of immunoprotective activity. Infect. Immun. 43: 442-444, 1984. PubMed: 6360908 4769: Alger NE, et al. Plasmodium berghei NK65 in the inbred A-J mouse: variations in virulence of P. berghei demes. J. Protozool. 18: 598-601, 1971. PubMed: 5133123 4843: Alger NE, Keshavarz-Valian H. Plasmodium berghei: the effects of suppressor factor on vaccination. Int. J. Parasitol. 14: 301-307, 1984. PubMed: 6381347 4893: D'Antonio LE, et al. Model system for study of artificially induced resistance to malaria. Nature 223: 507-509, 1969. PubMed: 5796953 5051: D'Antonio LE, et al. Malaria resistance: artificial induction with a partially purified plasmodial fraction. Science 168: 1117-1118, 1970. PubMed: 5441687 5060: D'Antonio LE. Plasmodium: a resume of the isolation of the vaccine fraction by the French pressure cell technique. Exp. Parasitol. 31: 75-81, 1972. PubMed: 5010607