ATCC® Number:50209™
Organism: Balamuthia mandrillaris Visvesvara et al.
Designations: CDC:V039
Isolation: brain tissue from 3 year, 10 month old female mandrill, Papio sphinx, that died of amebic meningoencephalitis, San Diego Zoo, 1986
Depositors: GS Visvesvara
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1156: RP mediumTemperature: 35.0°C Protocol: ATCCNO: 50209 SPEC: Upon arrival place frozen ampule directly into a 35C water bath, transfer thawed contents to 10 ml of fresh medium in a T-25 tissue culture flask containing a monolayer of African green monkey kidney cells (ATCCCRL-1586). The amoebae will completely destroy the monolayer and then encyst. Vigorously agitate the encysted culture and aseptically transfer 0.1 ml to a fresh monolayer of ATCCCRL-1586.
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Type Strain: yes(type strain)
Comments: growth in axenic media [32579] The amoebae can be maintained on a number of different cell lines [4990] agent of amebic meningoencephalitis [48914] [24186] experimental infection in mice [48915]
Classification: KINGDOM: Protozoa
References: 4990: Visvesvara GS, et al. Balamuthia mandrillaris, n. g., n. sp., agent of amebic meningoencephalitis in humans and other animals. J. Eukaryot. Microbiol. 40: 504-514, 1993. PubMed: 8330028 24186: Visvesvara GS, et al. Leptomyxid ameba, a new agent of amebic meningoencephalitis in humans and animals. J. Clin. Microbiol. 28: 2750-2756, 1990. PubMed: 2280005 32579: Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020 48914: Rideout BA, et al. Fatal infections with Balamuthia mandrillaris (a free-living amoeba) in gorillas and other Old World primates. Vet. Pathol. 34: 15-22, 1997. PubMed: 9150541 48915: Janitschke K, et al. Animal model Balamuthia mandrillaris CNS infection: contrast and comparison in immunodeficient and immunocompetent mice: a murine model of granulomatous amebic encephalitis. J. Neuropathol. Exp. Neurol. 55: 815-821, 1996. PubMed: 8965096
