ATCC? Number:50211?
Organism: Crithidia flexonema Wallace et al.
Isolation: water strider, Gerris remigis, Ames, IA, 1960
Depositors: LS Diamond
History: ATCC<<--LS Diamond<<--F.G. Wallace <<--- T.B. Clark
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Riboprinting and taxonomy [23607] Flagellates from genus Gerris [4693] Multiple distinct site-specific elements in miniexon arrays [24177]
References: 4693: Wallace FG, et al. Two new species of flagellates cultivated from insects of the genus Gerris. J. Protozool. 7: 390-393, 1960. 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515