ATCC? Number:50159?
Organism: Leishmania amazonensis Lainson and Shaw
Designations: IFLA/BR/67/PH8
Isolation: phlebotomine fly, Lutzomyia flaviscutellata, Belem, Para, Brazil, 1976
Depositors: WRAIR
History: ATCC<<--WRAIR<<--R. Lainson/J.J. Shaw
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 807: Brain heart infusion blood agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: WHO reference strain. Promastigotes.
References: 4655: Al-Taqi M, Evans DA. Characterization of Leishmania spp. from Kuwait by isoenzyme electrophoresis. Trans. R. Soc. Trop. Med. Hyg. 72: 56-65, 1978. PubMed: 635978 4920: Miles MA, et al. Some methods for the enzymic characterization of Latin-American Leishmania with particular reference to Leishmania mexicana amazonensis and subspecies of Leishmania hertigi. Trans. R. Soc. Trop. Med. Hyg. 74: 243-252, 1980. PubMed: 7385303