ATCC? Number:50174?
Organism: Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
Designations: RH
Isolation: 6-year-old male Homo sapiens, Cincinnati, OH, 1939
Depositors: RG Yaeger
History: ATCC<<--RG Yaeger<<--J. Remington <<--- W. Lunde ? <<--- A.B. Sabin
Biosafety Level:2
Shipped: frozen
Growth Conditions: Growth condition: in vivo, mouse Protocol: Inject the entire contents of the thawed ampule intraperitoneally into a 6- to 9-week-old mouse. The infection should be well developed within 6-9 days post inoculation. The abdomen of the mouse will become increasingly swollen as the infection progresses. When the infection is well developed, remove the peritoneal fluid from the mouse using the following technique: Inject 2 ml of Tyrode's solution into the peritoneum; massage the stomach for 2 minutes, and then using a 5 ml syringe, remove the peritoneal fluid (approximately 3 ml). Inoculate 0.2 ml of this fluid per mouse to subculture. TYRODES SALT SOLUTION: NaCl, 8.0 g KCl, 0.2 g CaCl2, 0.2 g MgCl2 . H2O, 0.05 g NaH2PO4 . H2O, 1.0 g NaHCO3, 1.0 g Glucose, 1.0 g Distilled H2O to, 1.0 L Filter sterilize.
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Comments: Host, strain and treatment variation as factors in the pathogenesis [23791] Toxoplasma hemagglutination and dye test antibodies [23805] Genome sequencing strain (in progress Broad Institute)
Related Items: purified DNA: ATCC50174DIn vitro strain: ATCCPRA-310
Classification: KINGDOM: Protozoa
References: 23791: Frenkel JK. Host, strain and treatment variation as factors in the pathogenesis of toxoplasmosis. Am. J. Trop. Med. Hyg. 2: 390-415, 1953. PubMed: 13040675 23805: Lunde MN, Jacobs L. Toxoplasma hemagglutination and dye test antibodies in experimentally infected rats. J. Parasitol. 49: 932-936, 1963. PubMed: 14084199