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Toxoplasma gondii
    Toxoplasma gondii
  • 平臺編號:bio-47782
  • 國際編號:ATCC 50174
  • 拉丁屬名: Toxoplasma gondii
  • 規(guī)格:frozen
  • 用途:ATCC原裝進口
  • 服務(wù)費用:
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  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

ATCC? Number:50174?
Organism: Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
Designations: RH
Isolation: 6-year-old male Homo sapiens, Cincinnati, OH, 1939
Depositors: RG Yaeger
History: ATCC<<--RG Yaeger<<--J. Remington <<--- W. Lunde ? <<--- A.B. Sabin
Biosafety Level:2
Shipped: frozen
Growth Conditions: Growth condition: in vivo, mouse Protocol: Inject the entire contents of the thawed ampule intraperitoneally into a 6- to 9-week-old mouse. The infection should be well developed within 6-9 days post inoculation. The abdomen of the mouse will become increasingly swollen as the infection progresses. When the infection is well developed, remove the peritoneal fluid from the mouse using the following technique: Inject 2 ml of Tyrode's solution into the peritoneum; massage the stomach for 2 minutes, and then using a 5 ml syringe, remove the peritoneal fluid (approximately 3 ml). Inoculate 0.2 ml of this fluid per mouse to subculture. TYRODES SALT SOLUTION: NaCl, 8.0 g KCl, 0.2 g CaCl2, 0.2 g MgCl2 . H2O, 0.05 g NaH2PO4 . H2O, 1.0 g NaHCO3, 1.0 g Glucose, 1.0 g Distilled H2O to, 1.0 L Filter sterilize.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Host, strain and treatment variation as factors in the pathogenesis [23791] Toxoplasma hemagglutination and dye test antibodies [23805] Genome sequencing strain (in progress Broad Institute)
Related Items: purified DNA: ATCC50174DIn vitro strain: ATCCPRA-310
Classification: KINGDOM: Protozoa
References: 23791: Frenkel JK. Host, strain and treatment variation as factors in the pathogenesis of toxoplasmosis. Am. J. Trop. Med. Hyg. 2: 390-415, 1953. PubMed: 13040675 23805: Lunde MN, Jacobs L. Toxoplasma hemagglutination and dye test antibodies in experimentally infected rats. J. Parasitol. 49: 932-936, 1963. PubMed: 14084199

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