ATCC? Number:50156?
Organism: Leishmania mexicana (Biagi) Garnham
Designations: MNYC/BZ/62/M379
Isolation: Nyctomys sumichrasti, Cayo District, Belize, 1962.
Depositors: WRAIR
History: ATCC<<--WRAIR<<--R. Lainson/Dr. Strangeways-Dixon
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 807: Brain heart infusion blood agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: WHO reference strain. Promastigotes. Selective killing of amastigotes [21691]
References: 4656: Evans DA, et al. The isolation and isoenzyme characterization of Leishmania braziliensis subsp. from patients with cutaneous leishmaniasis acquired in Belize. Trans. R. Soc. Trop. Med. Hyg. 78: 35-42, 1984. PubMed: 6710574 5169: D'Antonio LE, et al. Biochemical taxonomy of Leishmania. I. Observations on DNA. Ann. Trop. Med. Parasitol. 68: 307-316, 1974. PubMed: 4447389 21691: Muyombwe A, et al. Selective killing of Leishmania amastigotes expressing a thymidine kinase suicide gene. Exp. Parasitol. 85: 35-42, 1997. PubMed: 9024200