ATCC? Number:50127?
Organism: Leishmania donovani (Laveran and Mesnil) Ross
Designations: MHOM/ET/67/HU3
Isolation: human, Begemender, Ethiopia, 1965
Depositors: WRAIR
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 807: Brain heart infusion blood agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: WHO reference strain. Promastigotes.
References: 5163: Chance ML, et al. The biochemical and serological taxonomy of Leishmania from the Aethiopian zoogeographical region of Africa. Ann. Trop. Med. Parasitol. 72: 533-542, 1978. PubMed: 736662 5166: Jacobson RL, et al. Surface reaction of Leishmania. I. Lectin-mediated agglutination. Ann. Trop. Med. Parasitol. 76: 45-52, 1982. PubMed: 7082078 5170: Ann. Trop. Med. Parasitol. 75: 131-144, 1981.