ATCC? Number:50126?
Organism: Leishmania guyanensis Floch
Designations: MHOM/BR/75/M4147
Isolation: human, Brazil, 1975
Depositors: WRAIR
History: ATCC<<--WRAIR<<--R. Lainson/J.J. Shaw
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 807: Brain heart infusion blood agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: WHO reference strain. Promastigotes.
References: 4921: Miles MA, et al. Leishmaniasis in Brazil: XV. Biochemical distinction of Leishmania mexicana amazonensis, L. braziliensis braziliensis and L. braziliensis guyanensis--aetiological agents of cutaneous leishmaniasis in the Amazon Basin of Brazil. Trans. R. Soc. Trop. Med. Hyg. 75: 524-529, 1981. PubMed: 7324127