ATCC? Number:50118?
Organism: Crithidia sp.
Designations: Pip
Isolation: Photinus pyralis gut, Beltsville, MD, 1984
Depositors: TB Clark
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Multiple distinct site-specific elements in miniexon arrays [24177]
References: 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515