ATCC? Number:50115?
Organism: Trypanosoma cruzi Chagas
Designations: 2380-260
Isolation: raccoon, Procyon lotor, Laurel, MD, 1955
Depositors: LS Diamond
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1029: LIT mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Host specificity of ribosomal DNA variation [24058]
Classification: KINGDOM: Protozoa
References: 4703: Walton BC, et al. The isolation and identification of Trypanosoma cruzi from raccoons in Maryland. Am. J. Trop. Med. Hyg. 7: 603-610, 1958. PubMed: 13595203 4712: Diamond LS, Rubin R. Experimental infection of certain farm mammals with a North American strain of Trypanosoma cruzi from the raccoon. Exp. Parasitol. 7: 383-390, 1958. PubMed: 13562102 24058: Clark CG, Pung OJ. Host specificity of ribosomal DNA variation in sylvatic Trypanosoma cruzi from North America. Mol. Biochem. Parasitol. 66: 175-179, 1994. PubMed: 7984184