ATCC® Number:50124™
Organism: Leishmania aristedesi (Lainson and Shaw) emend Lainson and Shaw
Designations: MORY/PA/68/GML3
Isolation: Oryzomys capito, Sasardi, Darien, Panama, 1968
Depositors: WRAIR
History: ATCC<<--WRAIR<<--J.J. Shaw <<--- A. Herrer/S. Telford/H.A. Christensen
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 807: Brain heart infusion blood agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: WHO reference strain. Promastigotes.
References: 5168: Herrer A, et al. Enzootic cutaneous leishmaniasis in Eastern Panama. I. Investigation of the infection among forest mammals. Ann. Trop. Med. Parasitol. 65: 349-359, 1971. PubMed: 4999520 24415: Christensen HA, et al. The ecology of cutaneous Leishmaniasis in the republic of Panama. J. Med. Entomol. 20: 463-484.. PubMed: 6358496
