ATCC? Number:50112?
Organism: Prototheca ulmea Pore
Designations: RSP-1275
Isolation: American elm, Ulmus americana, Pennsylvania, 1984
Depositors: RS Pore
Biosafety Level:1
Shipped: freeze-dried
Growth Conditions: ATCCmedium 1371: Prototheca isolation medium (PIM)Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. Once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface.
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Type Strain: yes(type strain)
Classification: KINGDOM: Plantae
References: 20902: Pore RS. The association of Prototheca spp. with slime flux in Ulmus americana and other trees. Mycopathologia 94: 67-73, 1986.