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平臺編號：bio-47838
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國際編號：ATCC 50083
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拉丁屬名： Crithidia fasciculata
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Crithidia fasciculata
ATCC? Number:50083?
Organism: Crithidia fasciculata Leger
Designations: ReF-1:PRR
Isolation: cog-wheel bug, Arilus cristatus, Dorchester Co., MD, 1958
Depositors: LS Diamond
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1373: TTYSH mediumATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: susceptibility testing iodoquinol [23922] susceptibility testing metronidazole [23922] susceptibility testing paromomycin aminosidine, catenulin [23922] susceptibility testing tetracycline [23922]
Comments: Used in monoxenic culture and as an intermediate step in axenization of Entamoeba. Improved method for the monoxenic cultivation [4964] Riboprinting and taxonomy [23607] Use as food in monoxenic culture [24239] Axenic cultivation [23886] Multiple distinct site-specific elements in miniexon arrays [24177]
References: 4950: Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 4964: Diamond LS. Improved method for the monoxenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae with trypanosomatids. J. Parasitol. 54: 715-719, 1968. PubMed: 4319344 4985: Raether W, et al. Adaption of amoebae-Crithidia-cultures (Entamoeba histolytica) to axenic conditions of cultivation in TP-S-1-medium of Diamond 1968 (author's transl). Z. Parasitenkd. 42: 279-291, 1973. PubMed: 4360330 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23886: Clark CG. Axenic Cultivation of Entamoeba dispar Brumpt 1925, Entamoeba insolita Geiman and Wichterman 1937 and Entamoeba ranarum Grassi 1879. J. Eukaryot. Microbiol. 42: 590-593, 1995. PubMed: 7581333 23922: Chan FT, et al. Susceptibility testing of Dientamoeba fragilis ATCC30948 with iodoquinol, paromomycin, tetracycline, and metronidazole. Antimicrob. Agents Chemother. 38: 1157-1160, 1994. PubMed: 8067755 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 24239: Clark CG, et al. Entamoeba histolytica: is conversion of nonpathogenic amebae to the pathogenic form a real phenomenon?. Exp. Parasitol. 74: 307-314, 1992. PubMed: 1582483 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198
Organism: Crithidia fasciculata Leger
Designations: ReF-1:PRR
Isolation: cog-wheel bug, Arilus cristatus, Dorchester Co., MD, 1958
Depositors: LS Diamond
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1373: TTYSH mediumATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: susceptibility testing iodoquinol [23922] susceptibility testing metronidazole [23922] susceptibility testing paromomycin aminosidine, catenulin [23922] susceptibility testing tetracycline [23922]
Comments: Used in monoxenic culture and as an intermediate step in axenization of Entamoeba. Improved method for the monoxenic cultivation [4964] Riboprinting and taxonomy [23607] Use as food in monoxenic culture [24239] Axenic cultivation [23886] Multiple distinct site-specific elements in miniexon arrays [24177]
References: 4950: Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 4964: Diamond LS. Improved method for the monoxenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae with trypanosomatids. J. Parasitol. 54: 715-719, 1968. PubMed: 4319344 4985: Raether W, et al. Adaption of amoebae-Crithidia-cultures (Entamoeba histolytica) to axenic conditions of cultivation in TP-S-1-medium of Diamond 1968 (author's transl). Z. Parasitenkd. 42: 279-291, 1973. PubMed: 4360330 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23886: Clark CG. Axenic Cultivation of Entamoeba dispar Brumpt 1925, Entamoeba insolita Geiman and Wichterman 1937 and Entamoeba ranarum Grassi 1879. J. Eukaryot. Microbiol. 42: 590-593, 1995. PubMed: 7581333 23922: Chan FT, et al. Susceptibility testing of Dientamoeba fragilis ATCC30948 with iodoquinol, paromomycin, tetracycline, and metronidazole. Antimicrob. Agents Chemother. 38: 1157-1160, 1994. PubMed: 8067755 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 24239: Clark CG, et al. Entamoeba histolytica: is conversion of nonpathogenic amebae to the pathogenic form a real phenomenon?. Exp. Parasitol. 74: 307-314, 1992. PubMed: 1582483 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198
