ATCC? Number:50111?
Organism: Diaphanoeca grandis Ellis
Designations: 1/19/82 NB (3)
Isolation: Narragansett Bay, RI, 1982
Depositors: PG Davis
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 1361: Marine flagellate mediumMax Temperature: 18.0°C Min Temperature: 4.0°C Duration: grown with mixed bacteria Protocol: ATCCNO: 50111 SPEC: Upon receipt, remove test tube from package and immediately place it between 4 and 18C. Do not allow it to reach room temperature. Also place a T-25 tissue culture flask containing 10 ml of fresh medium and 1 or 2 autoclaved rice grains at the same temperature. Allow the vessels to equilibrate for 2-3 hours and then aseptically transfer a 0.1 ml aliquot from the test tube to the flask. Maintain both culture vessels. Transfer every 14-21 days when maintained at 10C.
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Comments: phylogeny [4912] Phylogenetic analysis of SSU rRNA, LSU rRNA, tubA, and hsp-90 [16172870]
Classification: KINGDOM: Protozoa
References: 4912: Wainright PO, et al. Monophyletic origins of the metazoa: an evolutionary link with fungi. Science 260: 340-342, 1993. PubMed: 8469985 16172870: Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774