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平臺編號：bio-47894
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國際編號：ATCC 50611
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拉丁屬名： Toxoplasma gondii
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
Toxoplasma gondii
Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux 拉丁名ATCC 50611 編號Deposited As Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
Strain Designations 菌種別名 me49
Application 用途
Food and waterborne pathogen research
Opportunistic pathogen research
Biosafety Level 安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation Unknown
Product Format 提供形式 frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures:
2-8°C
Live Cultures:
See Protocols section for handling information
Type Strain 模式菌株 no
Genotype Haplogroup 2
Genome Sequenced Strain
Yes
Comments
Genome sequencing strain
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 35°C to 37°C
Cell Line: ATCC CRL-1634 (human foreskin fibroblasts) (Contact ATCC Sales to order)
Cryopreservation Harvest and Preservation
To harvest the Toxoplasma culture, detach any remaining tissue culture cells (infected and uninfected) by scraping the surface of the flask with a cell scraper.
Transfer the cell suspension (including parasites) to 15 mL plastic centrifuge tubes. Centrifuge at 1300 x g for 10 min.
Remove all but 0.5 mL of the supernatant from each tube, resuspend the cell pellets, and pool them to a single tube.
Pass the resulting cell suspension through a syringe equipped with a 27 gauge 1/2 in needle to break up any remaining cells. Adjust the parasite concentration to 2.0 - 4.0 x 107 cells/mL with fresh medium or PBS. NOTE: If the concentration of parasites is too low, centrifuge at 1300 x g for 10 min and resuspend in the volume of fresh medium or PBS required to yield the desired concentration.
Prepare a cryoprotective solution containing 15% (v) DMSO and 50% (v) HIFBS in fresh medium or PBS.
Mix the cell preparation and cryoprotective solution in equal portions. The final concentration will be 1.0 - 2.0 x 107 cells/mL, 7.5% DMSO, and 25% HIFBS. The time from the mixing of the cell preparation and cryoprotective solution to the start of the freezing process should be no less than 15 min. and no more than 30 min. NOTE: To prevent culture contamination, penicillin-streptomycin solution (ATCC 30-2300) may be added to a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 μg/mL streptomycin.
Dispense in 0.5 mL aliquots to 1.0-2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
Store frozen ampules in either the vapor or liquid phase of a nitrogen refrigerator.
To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after thawed.
Immediately after thawing, aseptically transfer contents to a T-25 tissue culture flask containing a fresh monolayer of ATCC
CRL-1634
cells and 10 mL ATCC 30-2002 with 3% (v) HIFBS.
Outgas the flask for 10 seconds with a 95% air, 5% CO2 gas mixture.
Incubate in a 35-37°C CO2 incubator with the cap screwed on tightly.
Name of Depositor LM Weiss
Special Collection NCRR Contract
References 參考文獻
Khan A, et al. Recent transcontinental sweep of Toxoplasma gondii driven by a single monomorphic chromosome. Proc. Natl. Acad. Sci. USA 104: 14872-14877, 2007. PubMed: 17804804
Cross References
Nucleotide (GenBank) : ABPA01000000 Toxoplasma gondii ME49, whole genome shotgun sequencing project.
Strain Designations 菌種別名 me49
Application 用途
Food and waterborne pathogen research
Opportunistic pathogen research
Biosafety Level 安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation Unknown
Product Format 提供形式 frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures:
2-8°C
Live Cultures:
See Protocols section for handling information
Type Strain 模式菌株 no
Genotype Haplogroup 2
Genome Sequenced Strain
Yes
Comments
Genome sequencing strain
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 35°C to 37°C
Cell Line: ATCC CRL-1634 (human foreskin fibroblasts) (Contact ATCC Sales to order)
Cryopreservation Harvest and Preservation
To harvest the Toxoplasma culture, detach any remaining tissue culture cells (infected and uninfected) by scraping the surface of the flask with a cell scraper.
Transfer the cell suspension (including parasites) to 15 mL plastic centrifuge tubes. Centrifuge at 1300 x g for 10 min.
Remove all but 0.5 mL of the supernatant from each tube, resuspend the cell pellets, and pool them to a single tube.
Pass the resulting cell suspension through a syringe equipped with a 27 gauge 1/2 in needle to break up any remaining cells. Adjust the parasite concentration to 2.0 - 4.0 x 107 cells/mL with fresh medium or PBS. NOTE: If the concentration of parasites is too low, centrifuge at 1300 x g for 10 min and resuspend in the volume of fresh medium or PBS required to yield the desired concentration.
Prepare a cryoprotective solution containing 15% (v) DMSO and 50% (v) HIFBS in fresh medium or PBS.
Mix the cell preparation and cryoprotective solution in equal portions. The final concentration will be 1.0 - 2.0 x 107 cells/mL, 7.5% DMSO, and 25% HIFBS. The time from the mixing of the cell preparation and cryoprotective solution to the start of the freezing process should be no less than 15 min. and no more than 30 min. NOTE: To prevent culture contamination, penicillin-streptomycin solution (ATCC 30-2300) may be added to a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 μg/mL streptomycin.
Dispense in 0.5 mL aliquots to 1.0-2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
Store frozen ampules in either the vapor or liquid phase of a nitrogen refrigerator.
To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after thawed.
Immediately after thawing, aseptically transfer contents to a T-25 tissue culture flask containing a fresh monolayer of ATCC
CRL-1634 cells and 10 mL ATCC 30-2002 with 3% (v) HIFBS.Outgas the flask for 10 seconds with a 95% air, 5% CO2 gas mixture.
Incubate in a 35-37°C CO2 incubator with the cap screwed on tightly.
Name of Depositor LM Weiss
Special Collection NCRR Contract
References 參考文獻
Khan A, et al. Recent transcontinental sweep of Toxoplasma gondii driven by a single monomorphic chromosome. Proc. Natl. Acad. Sci. USA 104: 14872-14877, 2007. PubMed: 17804804
Cross References
Nucleotide (GenBank) : ABPA01000000 Toxoplasma gondii ME49, whole genome shotgun sequencing project.
