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肺炎支原體
ATCC? Number:40750?
Organism: Crypthecodinium cohnii (Seligo) Javornicky
Designations: MK8805 [MK8840, UTEX 1649]
Depositors: Martek Biosciences Corporation, D Kyle, Martek Biosciences Corporation
History: ATCC<<--Martek Biosciences Corporation, D Kyle, Martek Biosciences Corporation<<--UTEX 1649
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 2076: Crypthecodinium mediumMax Temperature: 30.0℃ Min Temperature: 20.0℃ Duration: axenic Protocol: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCCmedium 2076 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen by one half turn, and incubate the culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times, aseptically transfer a 0.1 ml aliquot to 5 ml of ATCCmedium 2076, and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: produces a single-cell edible oil containing docohexaenoic acid [33738] [33740] [33739]
Classification: KINGDOM: Protozoa
References: 33738: Kyle DJ, et al. Infant formula and baby food containing docosahexaenoic acid obtained from dinoflagellates. US Patent 5,397,591 dated Mar 14 1995 33739: Kyle DJ, et al. Production of docosahexaenoic acid by dinoflagellates. US Patent 5,407,957 dated Apr 18 1995 33740: Kyle DJ, et al. Pharmaceutical composition and dietary supplement containing docosarexaenoic [docosahexaenoic] acid obtained from dinoflagellates. US Patent 5,492,938 dated Feb 20 1996