ATCC? Number:30983?
Organism: Tetramitus rostratus Bunting
Designations: Tr O
Isolation: bat guano
Depositors: W Balamuth
History: ATCC<<--W Balamuth<<--D. Outka
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30216 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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