ATCC? Number:40335?
Organism: Neospongiococcum excentricum Deason and Bold
Designations: HS1 [B]
Isolation: division mutant derived from wild type strain UTEX 1232, 1987
Depositors: Universal Foods Corp.
History: ATCC<<--Universal Foods Corp.<<--D.L. Heefner
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 2001: CYU 2%Max Temperature: 35.0°C Min Temperature: 25.0°C Protocol: ATCCNO: 40335 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCCmedium 2001 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen the cap one half turn, and incubate the flask on a horizontal slant (5-15 degree slant) at 25-35C. The culture should be ready to subculture in 5-7 days. To subculture, screw the cap on tightly, agitate vigorously and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCCmedium 2001. Incubate as above.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: produces carotenoids [4569]
Comments: Carotenoid producing culture [4569]
References: 4569: Liao HH, et al. Carotenoid producing culture using Nespongiococcum excentricum. US Patent 5,437,997 dated Aug 1 1995 31242: Orndorff SA, et al. Zeaxanthin producing strains of Neospongiococcum excentricum. US Patent 5,360,730 dated Nov 1 1994