ATCC? Number:30982?
Organism: Paramecium tetraurelia Sonneborn
Designations: stock d4-113
Isolation: d5-113 (a mutant derived from stock 51 genome subjected to X-ray by S. Igarashi, 1964) X d4-96 (pwB2 mutant induced by nitrosoguanidine by C. Kung, pre-1976), 1976
Depositors: JR Preer 
History: ATCC<<--JR Preer<<--T.M. Sonneborn
Biosafety Level:1
Shipped: test tube 
Growth Conditions: Protocol: ATCCNO: 30982 SPEC: This strain is shipped as a growing test tube culture.  Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap,  loosen the cap one half turn.  Add 1.0 ml of ATCCmedium 802 bacterized with Enterobacter aerogenes ATCC13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCCmedium 802 in a 20 x 120 mm test tube.  To retain the phenotype of nondischarge trichocysts, follow each feeding with 2-3 days starvation.
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Geno Type: pwB2
Comments: Line # 76M10-28A-4BS
Classification: KINGDOM: Protozoa
References:  4608: . .  Dev. Genet. 1: 21-46, 1979. 5160:  Handbook of genetics. vol. 2New York: Plenum Press; 1975.