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平臺(tái)編號(hào)：bio-47957
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國(guó)際編號(hào)：ATCC 40318
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拉丁屬名： Trichosphaerium sp.
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規(guī)格：test tube
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用途：固氮
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Trichosphaerium sp.
ATCC? Number:40318?
Organism: Trichosphaerium sp.
Designations: Am-I-7 wt (Amoeba-I-7 Wild Type)
Isolation: stipes of Sargassum muticum, Alegria Beach, Hollister Ranch, Santa Barbara Co., CA, 1984
Depositors: M Polne-Fuller
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 1405: HESNW mediumMax Temperature: 25.0°C Min Temperature: 20.0°C Protocol: ATCCNO: 40318 SPEC: This strain is shipped as a test tube culture (10 ml of culture in a 20 x 125-mm screw-capped test tube). Upon arrival, rub the internal surfaces of the tube with a sterile cotton swab to dislodge any adhering amoebae. Aseptically divide the entire contents into 2 equal portions and distribute to T-25 tissue culture flasks. Add 5 ml of fresh ATCCmedium 1405 and 0.5 ml of heat-killed Dunaliella tertiolecta ATCC30929 to each flask. Screw the caps on tightly and incubate at 20-25C. Transfer every 4-6 weeks. Rub the surface of the flask to be subcultured with a sterile cotton swab, agitate and aseptically transfer a 0.25-0.5 ml aliquot to a fresh flask containing 10 ml of ATCCmedium 1405 supplemented with 0.5 ml of heat-killed algal suspension. Incubate as above. Note: Since this strain is cultivated with heat-killed Dunaliella tertiolecta ATCC30929 as a food source, the food should be prepared before the strain is ordered. The food is prepared as follows: 1. Establish a culture of ATCC30929 in 5 ml of ATCCmedium 1194 broth in a 16 x 125-mm screw-capped test tube. Incubate with the cap on loose under 50-75 (Einsteins/m2/s of light at 25C. 2. When the culture reaches early stationary phase, aseptically transfer 0.25-ml aliquots to each of 10 250-ml cotton-plugged Erlenmeyer flasks containing 100 ml of ATCCmedium 1194 broth. Incubate as above. 3. When the cultures reach early stationary phase (approximately 10-14 days), harvest as follows: Aseptically transfer algal suspensions to 50-ml plastic centrifuge tubes. Centrifuge at 400-500 x g for 5 minutes. 4. Decant supernatant and resuspend each pellet in approximately one ml of ATCCmedium 1405. Pool all suspensions in a single centrifuge tube and centrifuge as in step 3. The cell pellet should be approximately 5 ml. 5. Resuspend the cell pellet to a final volume of approximately 14 ml with ATCCmedium 1405 and transfer to a 15-ml plastic centrifuge tube. Centrifuge as in step 3. Discard the supernatant. 6. Resuspend the cell pellet to a final volume of approximately 14 ml with ATCCmedium 1405 and centrifuge as in step 3. Discard the supernatant. 7. Resuspend the final cell pellet to a final volume of 10 ml with ATCCmedium 1405. Transfer the cell suspension to a sterile 125-ml screw-capped bottle and aseptically add 40 ml of ATCCmedium 1405 bringing the final volume to 50 ml.WARN: Last ATCC#: 40318, unprocessed line: 8. Place bottle prepared in step 7 in a 60C water bath to a level such that the liquid level of the water bath is above that of the suspension in the bottle. Incubate for a total of 30 minutes, swirling the bottle at 10-minute intervals. Allow the bottle to cool to room temperature. This treatment will kill all algal cells. 9. As a check for viable cells, add 3 drops of the cell suspension prepared in step 10 to the edge of a 100-mm petri plate containing ATCCmedium 1194 agar. Hold the plate vertically to allow the drops to move to the opposite edge. Incubate plate at 25C under 50-75 uE of light for 7 days. 10. The heat-killed algae can be stored at 4C for up to one year.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: microorganisms for degrading plant cell walls [4568]
Comments: degradation of plant cell walls [4568] digestion of seaweeds [4812]
Classification: KINGDOM: Protozoa
References: 4568: Polne-Fuller M. Microoganisms and methods for degrading plant cell walls and complex hydrocarbons. US Patent 5,413,933 dated May 9 1995 4812: Polne-Fuller M. A multinucleated marine amoeba which digests seaweeds. J. Protozool. 34: 159-165, 1987.
Organism: Trichosphaerium sp.
Designations: Am-I-7 wt (Amoeba-I-7 Wild Type)
Isolation: stipes of Sargassum muticum, Alegria Beach, Hollister Ranch, Santa Barbara Co., CA, 1984
Depositors: M Polne-Fuller
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 1405: HESNW mediumMax Temperature: 25.0°C Min Temperature: 20.0°C Protocol: ATCCNO: 40318 SPEC: This strain is shipped as a test tube culture (10 ml of culture in a 20 x 125-mm screw-capped test tube). Upon arrival, rub the internal surfaces of the tube with a sterile cotton swab to dislodge any adhering amoebae. Aseptically divide the entire contents into 2 equal portions and distribute to T-25 tissue culture flasks. Add 5 ml of fresh ATCCmedium 1405 and 0.5 ml of heat-killed Dunaliella tertiolecta ATCC30929 to each flask. Screw the caps on tightly and incubate at 20-25C. Transfer every 4-6 weeks. Rub the surface of the flask to be subcultured with a sterile cotton swab, agitate and aseptically transfer a 0.25-0.5 ml aliquot to a fresh flask containing 10 ml of ATCCmedium 1405 supplemented with 0.5 ml of heat-killed algal suspension. Incubate as above. Note: Since this strain is cultivated with heat-killed Dunaliella tertiolecta ATCC30929 as a food source, the food should be prepared before the strain is ordered. The food is prepared as follows: 1. Establish a culture of ATCC30929 in 5 ml of ATCCmedium 1194 broth in a 16 x 125-mm screw-capped test tube. Incubate with the cap on loose under 50-75 (Einsteins/m2/s of light at 25C. 2. When the culture reaches early stationary phase, aseptically transfer 0.25-ml aliquots to each of 10 250-ml cotton-plugged Erlenmeyer flasks containing 100 ml of ATCCmedium 1194 broth. Incubate as above. 3. When the cultures reach early stationary phase (approximately 10-14 days), harvest as follows: Aseptically transfer algal suspensions to 50-ml plastic centrifuge tubes. Centrifuge at 400-500 x g for 5 minutes. 4. Decant supernatant and resuspend each pellet in approximately one ml of ATCCmedium 1405. Pool all suspensions in a single centrifuge tube and centrifuge as in step 3. The cell pellet should be approximately 5 ml. 5. Resuspend the cell pellet to a final volume of approximately 14 ml with ATCCmedium 1405 and transfer to a 15-ml plastic centrifuge tube. Centrifuge as in step 3. Discard the supernatant. 6. Resuspend the cell pellet to a final volume of approximately 14 ml with ATCCmedium 1405 and centrifuge as in step 3. Discard the supernatant. 7. Resuspend the final cell pellet to a final volume of 10 ml with ATCCmedium 1405. Transfer the cell suspension to a sterile 125-ml screw-capped bottle and aseptically add 40 ml of ATCCmedium 1405 bringing the final volume to 50 ml.WARN: Last ATCC#: 40318, unprocessed line: 8. Place bottle prepared in step 7 in a 60C water bath to a level such that the liquid level of the water bath is above that of the suspension in the bottle. Incubate for a total of 30 minutes, swirling the bottle at 10-minute intervals. Allow the bottle to cool to room temperature. This treatment will kill all algal cells. 9. As a check for viable cells, add 3 drops of the cell suspension prepared in step 10 to the edge of a 100-mm petri plate containing ATCCmedium 1194 agar. Hold the plate vertically to allow the drops to move to the opposite edge. Incubate plate at 25C under 50-75 uE of light for 7 days. 10. The heat-killed algae can be stored at 4C for up to one year.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: microorganisms for degrading plant cell walls [4568]
Comments: degradation of plant cell walls [4568] digestion of seaweeds [4812]
Classification: KINGDOM: Protozoa
References: 4568: Polne-Fuller M. Microoganisms and methods for degrading plant cell walls and complex hydrocarbons. US Patent 5,413,933 dated May 9 1995 4812: Polne-Fuller M. A multinucleated marine amoeba which digests seaweeds. J. Protozool. 34: 159-165, 1987.
