ATCC® Number:30973™
Organism: Acanthamoeba rhysodes (Singh) Singh and Hanumaiah
Designations: HrB
Isolation: soil, Rothamsted Experimental Station, Harpenden, England, 1952
Depositors: W Balamuth
History: ATCC<<--W Balamuth<<--B.N. Singh
Biosafety Level:1
Shipped: Frozen
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Genetic markers that distinguish pathogenic from nonpathogenic strains [31825] mitochondrial DNA fingerprinting [21722] Subgenus systematics based upon SSU rDNA sequence data [21640] PCR for Identifiction of Naegleria fowleri [23815] characterization of Acanthamoeba polyphaga [24209] review [23576] phylogeny
Classification: KINGDOM: Protozoa
References: 21640: Gast RJ, et al. Subgenus systematics of Acanthamoeba: Four nuclear 18S rDNA sequence types. J. Eukaryot. Microbiol. 43: 498-504, 1996. PubMed: 8976608 21722: Gautom RK, et al. Mitochondrial DNA fingerprinting of Acanthamoeba spp. isolated from clinical and environmental sources. J. Clin. Microbiol. 32: 1070-1073, 1994. PubMed: 7913095 23576: John DTOpportunistically pathogenic free-living amebaeIn: John DTParasitic protozoa2nd ed.3San DiegoAcademic Presspp. 143-246, 1993 23815: Kilvington S, Beeching J. Development of a PCR for identification of Naegleria fowleri from the environment. Appl. Environ. Microbiol. 61: 3764-3767, 1995. PubMed: 7487014 23877: Singh BN. Nuclear division in nine species of small free-living amoebae and its bearing on the classification of the order amoebida. Philos. Trans. R. Soc. Lond. B Biol. Sci. London., 2: 405-460, 1951. 24209: Fritsche TR, et al. Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses. J. Clin. Microbiol. 31: 1122-1126, 1993. PubMed: 8501212 31825: Howe D, et al. Identification of two genetic markers that distinguish pathogenic and nonpathogenic strains of Acanthamoeba spp.. Parasitol. Res. 83: 435-348, 1997. PubMed: 9197389 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 43732: Stothard DR, et al. Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type. J. Clin. Microbiol. 37: 2687-2693, 1999. PubMed: 10405422 57068: Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331 71069: Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev. 16: 273-307, 2003. PubMed: 12692099
ATCC30973
