Naegleria australiensis De Jonckheere 拉丁名
(ATCC® 30958™) 統(tǒng)一編號(hào)
Strain Designations PP 397
Application 用途 Production of monoclonal antibodies
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Isolation flood drainage water, South Australia, 1973
Product Format 提供形式 frozen
Type Strain 模式菌株 yes
Comments 注釋 Zymodeme Au-1； Maximum temperature tolerance 42C；
Isoenzyme electrophoresis；
species description；
Restriction fragment length polymorphisms of DNA；
Interrepeat PCR；
Thermal ecology；
Comparative biochemical study；
High-resolution (PGGE) of isoenzymes；
Production of monoclonal antibodies；
Occurrence in natural waters in Mexico；
Five cases of PAM；
Medium 培養(yǎng)基 ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Growth Conditions 省這條街
Temperature 培養(yǎng)溫度: 35.0°C
Duration: axenic
Cryopreservation 1. Harvest cells from a culture which is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
2. Adjust the concentration of cells to 2.0 x 106/ml.
If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
3. Prepare a 10% (v/v) sterile DMSO solution in ATCC medium 1034 as follows: Add the required volume of DMSO to a glass screw-capped test tube and place on ice. Allow the DMSO to solidify. Add the required volume of refrigerated ATCC medium 1034. Dissolve the DMSO by inverting several times. If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 60 min.
5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.
9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 5.0 ml of fresh ATCC medium 1034.
10. Incubate the tube on a 15° horizontal at 35°C with the cap screwed on tightly.Name of Depositor JF De Jonckheere
Chain of Custody ATCC <<--JF De Jonckheere<<--E. Willaert <<--- A. Jamieson
Year of Origin 1973
References De Jonckheere JF, et al. A comparative study of 14 strains of Naegleria australiensis demonstrates the existence of a highly virulent subspecies: N. australiensis italica n. spp. J. Protozool. 31: 324-331, 1984. PubMed: 6470990
De Jonckheere. Naegleria australiensis sp. nov., another pathogenic Naegleria from water. Protistologica 17: 423-429, 1981.
Environ. Res. 59: 223-226, 1992.
McLaughlin GL, et al. Restriction fragment length polymorphisms of the DNA of selected Naegleria and Acanthamoeba amebae. J. Clin. Microbiol. 26: 1655-1658, 1988. PubMed: 2903176
Carosi G, et al. Ultrastructure of Naegleria australiensis (pp-397 strain) with particular regard to pleomorphic cytoplasmic RER-associated inclusions. Protistologica 22: 169-174, 1986.
van Belkum A, et al. Genotyping Naegleria spp. and Naegleria Fowleri isolates by Interepeat Polymerase Chain reaction. J. Clin. Microbiol. 30: 2595-2598, 1992. PubMed: 1400959
Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158
De Jonckheere JF. Variation of electrophoretic karyotypes among Naegleria spp.. Parasitol. Res. 76: 55-62, 1989. PubMed: 2622896
Huizinga HW, McLaughlin GL. Thermal ecology of Naegleria fowleri from a power plant cooling reservoir. Appl. Environ. Microbiol. 56: 2200-2205, 1990. PubMed: 1975164
Hadas E, Kasprzak W. Comparative biochemical studies on the pathogenic and non-pathogenic amebae of the genus Naegleria. Wiad. Parazytol. 33: 25-38, 1987. PubMed: 3307159
Moss DM, et al. High-resolution polyacrylamide gradient gel electrophoresis (PGGE) of isoenzymes from five Naegleria species. J. Protozool. 35: 26-31, 1988. PubMed: 2966859
Visvesvara GS, et al. Production of monoclonal antibodies to Naegleria fowleri, agent of primary amebic meningoencephalitis. J. Clin. Microbiol. 25: 1629-1634, 1987. PubMed: 3308948
Rivera F, et al. Pathogenic amoebae in natural thermal waters of three resorts of Hidalgo, Mexico. Environ. Res. 50: 289-295, 1989. PubMed: 2583075
Lares-Villa F, et al. Five cases of primary amebic meningoencephalitis in Mexicali, Mexico: study of the isolates. J. Clin. Microbiol. 31: 685-688, 1993. PubMed: 8458963
Rivera F, et al. Contaminacion del liquido cefolorraquideo de un infante con sindrome de Arnold-Chiari tipo II, hidrocefalia y mielomeningocele, por Naegleria lovaniensis. Rev. Enferm. Infecc. Pediatr. 2: 91-94, 1989. Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360
De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.
Reveiller FL, et al. Species specificity of a monoclonal antibody produced to Naegleria fowleri and partial characterization of its antigenic determinant. Parasitol. Res. 86: 634-641, 2000. PubMed: 10952262
Reveiller FL, et al. Isolation of a unique membrane protein from Naegleria fowleri. J. Eukaryot. Microbiol. 48: 676-682, 2001. PubMed: 11831777
