ATCC? Number:30920?
Organism: Colpoda steinii Maupas
Isolation: dried grass, Montezuma, NY, 1980
Depositors: DH Lynn
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Duration: grown with bacteria Protocol: ATCCNO: 30916 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in a T-25 tissue culture flask containing 10 ml ATCCmedium 802 inoculated 24 hours previously with Enterobacter aerogenes ATCC13048. Transfer any residual liquid from the vial to the flask. Place at 25C. Excystment should occur within a few days.
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Applications: testing
Classification: KINGDOM: Protozoa
References: 23845: Wickham SA, Lynn DH. Relations between growth rate, cell size, and DNA content in Colpodean Ciliates (Ciliophora: Colpodea). Eur. J. Protistol. 25: 345-352, 1990. 32134: Nanney DL, et al. Comparison of sequence differences in a variable 23S rRNA domain among sets of cryptic species of ciliated protozoa. J. Eukaryot. Microbiol. 45: 91-100, 1998. PubMed: 9495037