ATCC? Number:30917?
Organism: Colpoda inflata (Stokes) Kahl
Isolation: dried hay, Toronto, Ontario, Canada, 1970
Depositors: DH Lynn
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Duration: grown with bacteria Protocol: ATCCNO: 30916 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in a T-25 tissue culture flask containing 10 ml ATCCmedium 802 inoculated 24 hours previously with Enterobacter aerogenes ATCC13048. Transfer any residual liquid from the vial to the flask. Place at 25C. Excystment should occur within a few days.
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Applications: testing [92300]
Comments: Phylogenetic relationships [4596]
Cross References: Nucleotide (GenBank) : M97908 Colpoda inflata small subunit ribosomal RNA gene sequence.
Classification: KINGDOM: Protozoa
References: 4596: Greenwood SJ, et al. Phylogenetic relationships of Blepharisma americanum and Colpoda inflata within the phylum ciliophora inferred from complete small subunit rRNA gene sequences. J. Protozool. 38: 1-6, 1991. PubMed: 1900085 23845: Wickham SA, Lynn DH. Relations between growth rate, cell size, and DNA content in Colpodean Ciliates (Ciliophora: Colpodea). Eur. J. Protistol. 25: 345-352, 1990. 23946: Bowers NJ, Pratt JR. Estimation of genetic variation among soil isolates of colpoda inflata (Stokes) (Protozoa: Ciliophora) using the polymerase chain restriction fragment length polymorphism analysis. Arch. Protistenkd. 145: 29-36, 1995. 92300: 8310 D: Growth inhibition test with the soil ciliate Colpoda inflata. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA8310 - Ciliated Protozoa;.