ATCC? Number:30901?
Organism: Acanthamoeba astronyxis (Ray and Hayes) Page
Designations: Res 20
Isolation: pond, Madison, WI, 1964
Depositors: FC Page
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Re-definition of the genus [4757] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 4757: Page FC. Re-definition of the genus Acanthamoeba with descriptions of three species. J. Protozool. 14: 709-724, 1967. PubMed: 5604481 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 70201: Alves JMP, et al. Random amplified polymorphic DNA probes as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba. Braz. J. Med. Biol. Res. 33: 19-26, 2000.