ATCC? Number:30899?
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: CCAP 1501/3H
Isolation: respiratory swab from child, Syracuse, NY, 1959
Depositors: CCAP
History: ATCC<<--CCAP<<--R. Duma <<--- S.L. Chang <<--- S. Wang
Biosafety Level:2
Shipped: freeze-dried
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30010 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
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Comments: Experimental pneumonitis and encephalitis [5020] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 5020: Martinez AJ, et al. Experimental pneumonitis and encephalitis caused by Acanthamoeba in mice: pathogenesis and ultrastructural features. J. Infect. Dis. 131: 692-699, 1975. PubMed: 48530 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681