ATCC? Number:30879?
Organism: Leishmania braziliensis Vianna
Designations: 1077
Isolation: human male forearm skin lesion, Ft Sherman, Panama Canal Zone, 1974
Depositors: L Hendricks
History: ATCC<<--L Hendricks<<--E. Murray <<--- L. Hendricks
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 431: Trypanosome mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Promastigotes. Karyotype analysis [4910]
References: 4910: Giannini SH, et al. Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis. Science 232: 762-765, 1986. PubMed: 3961502