ATCC? Number:30898?
Organism: Acanthamoeba castellanii (Douglas) Page
Designations: CCAP 1501/2B
Isolation: well, Cincinnati, OH, 1959
Depositors: CCAP
History: ATCC<<--CCAP<<--R.J. Duma <<--- . . . <<--- S.L. Chang
Biosafety Level:2
Shipped: freeze-dried
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30010 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
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Comments: Experimental pneumonitis and encephalitis [5020] Biochemical and molecular characterization [21721] DNA, RNA and Protein Sequences [24022] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 5020: Martinez AJ, et al. Experimental pneumonitis and encephalitis caused by Acanthamoeba in mice: pathogenesis and ultrastructural features. J. Infect. Dis. 131: 692-699, 1975. PubMed: 48530 21721: Chung DI, et al. Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil. Korean J. Parasitol. 34: 79-85, 1996. PubMed: 8820744 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24022: Byers TJ, et al. Genes of Acanthamoeba: DNA, RNA and protein sequences (a review). J. Protozool. 37: 17S-25S, 1990. PubMed: 1701831 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 57068: Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331 70752: Reveiller FL, et al. Species specificity of a monoclonal antibody produced to Naegleria fowleri and partial characterization of its antigenic determinant. Parasitol. Res. 86: 634-641, 2000. PubMed: 10952262