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平臺(tái)編號(hào)：bio-48039
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國際編號(hào)：ATCC 30877
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拉丁屬名： Naegleria gruberi
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規(guī)格：dried
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Naegleria gruberi
ATCC? Number:30877?
Organism: Naegleria gruberi Schardinger
Designations: CCAP 1518/1g
Isolation: freshwater, Tuskegee, AL, 1965
Depositors: CCAP
History: ATCC<<--CCAP<<--F.C. Page 48
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Zymodeme Gr-10 [23932] Maximum temperature tolerance 37C [23932] Equivalent to CCAP 1518/1g, maximum temperature tolerance 36C [38900] biochemical identification [4799] Genetic cluster E [Int. J. Parasitol. 19: 823-834, 1989] = Naegleria gruberi unknown cluster [J. Eukaryot. Microbiol. 40: 179-187, 1993]. Equivalent to CCAP 1518/1g, Group I intron in SSUrRNA gene [23861]
References: 4756: Page FC. Taxonomic criteria for limax amoebae, with descriptions of 3 new species of Hartmannella and 3 of Vahlkampfia. J. Protozool. 14: 499-521, 1967. PubMed: 6050658 4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4936: Page FC. Morphological variation in the cyst wall of Naegleria gruberi (amoebida, vahlkampfiidae). Protistologica 11: 195-204, 1975. 23861: De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 38900: Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360
Organism: Naegleria gruberi Schardinger
Designations: CCAP 1518/1g
Isolation: freshwater, Tuskegee, AL, 1965
Depositors: CCAP
History: ATCC<<--CCAP<<--F.C. Page 48
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Zymodeme Gr-10 [23932] Maximum temperature tolerance 37C [23932] Equivalent to CCAP 1518/1g, maximum temperature tolerance 36C [38900] biochemical identification [4799] Genetic cluster E [Int. J. Parasitol. 19: 823-834, 1989] = Naegleria gruberi unknown cluster [J. Eukaryot. Microbiol. 40: 179-187, 1993]. Equivalent to CCAP 1518/1g, Group I intron in SSUrRNA gene [23861]
References: 4756: Page FC. Taxonomic criteria for limax amoebae, with descriptions of 3 new species of Hartmannella and 3 of Vahlkampfia. J. Protozool. 14: 499-521, 1967. PubMed: 6050658 4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4936: Page FC. Morphological variation in the cyst wall of Naegleria gruberi (amoebida, vahlkampfiidae). Protistologica 11: 195-204, 1975. 23861: De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 38900: Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360
