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平臺編號：bio-48040
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國際編號：ATCC 30874
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拉丁屬名： Naegleria pringsheimi
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規(guī)格：dried
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Naegleria pringsheimi
ATCC? Number:30874?
Organism: Naegleria pringsheimi De Jonckheere
Designations: CCAP 1518/1a
Isolation: pre-1950
Depositors: CCAP
History: ATCC<<--CCAP<<--E.G. Pringsheim
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Zymodeme Gr-4 [23932] CCAP 15181/a is no longer available for CCAP Maximum temperature tolerance 37C [23932] genetic cluster M [38900] Genetic cluster M [RF34321] = Naegleria gruberi cluster 3 [RF34247]. Equivalent to CCAP 1518/1a, Group I intron in SSUrRNA gene [23861] Thermal ecology [24033] biochemical identification
References: 4936: Page FC. Morphological variation in the cyst wall of Naegleria gruberi (amoebida, vahlkampfiidae). Protistologica 11: 195-204, 1975. 23861: De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 24033: Huizinga HW, McLaughlin GL. Thermal ecology of Naegleria fowleri from a power plant cooling reservoir. Appl. Environ. Microbiol. 56: 2200-2205, 1990. PubMed: 1975164 38900: Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360 61465: De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.
ATCC30874
Organism: Naegleria pringsheimi De Jonckheere
Designations: CCAP 1518/1a
Isolation: pre-1950
Depositors: CCAP
History: ATCC<<--CCAP<<--E.G. Pringsheim
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Zymodeme Gr-4 [23932] CCAP 15181/a is no longer available for CCAP Maximum temperature tolerance 37C [23932] genetic cluster M [38900] Genetic cluster M [RF34321] = Naegleria gruberi cluster 3 [RF34247]. Equivalent to CCAP 1518/1a, Group I intron in SSUrRNA gene [23861] Thermal ecology [24033] biochemical identification
References: 4936: Page FC. Morphological variation in the cyst wall of Naegleria gruberi (amoebida, vahlkampfiidae). Protistologica 11: 195-204, 1975. 23861: De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 24033: Huizinga HW, McLaughlin GL. Thermal ecology of Naegleria fowleri from a power plant cooling reservoir. Appl. Environ. Microbiol. 56: 2200-2205, 1990. PubMed: 1975164 38900: Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360 61465: De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.
ATCC30874
